发明名称 PNA probes, mixtures, methods and kits pertaining to the determination of <i>Mycoplasma </i>and related <i>Mollicutes</i>
摘要 This invention is related to PNA probes, probe sets, mixtures, methods and kits pertaining to the determination of Mycoplasma and related Mollicutes.
申请公布号 US8753821(B2) 申请公布日期 2014.06.17
申请号 US201213400316 申请日期 2012.02.20
申请人 Applied Biosystems, LLC 发明人 Hyldig-Nielsen Jens;Rigby Susan;Tanner Michael;Lee Ditte;Brehm-Stecher Byron
分类号 C12Q1/68 主分类号 C12Q1/68
代理机构 代理人
主权项 1. A mixture or kit comprising: a) five nucleic acid primers each having a length of 23-30 nucleotides, wherein each of the five primers comprise one of SEQ ID Nos.: 1-5, each of which primer hybridizes to a sequence within the nucleic acid of one or more Mollicutes; and b) four PNA probes, each of which probe comprises a probing nucleobase sequence of 12-20 nucleobase containing subunits, wherein the probing nucleobase sequence is selected from the group consisting of SEQ. ID. NO: 6, SEQ. ID. NO: 7, SEQ. ID. NO: 8, SEQ. ID. NO: 9, the complement to SEQ. ID. NO: 6, the complement to SEQ. ID. NO: 7, the complement to SEQ. ID. NO: 8 and the complement to SEQ. ID. NO: 9, and wherein each probe hybridizes to a target sequence within the nucleic acid of one or more Mollicutes.
地址 Carlsbad CA US