RECEPTOR TYROSINE KINASE ASSAYS

摘要

Methods for detecting phosphorylation of receptor tyrosine kinases ("RTKs") upon activation are provided. The method employs cells comprising two fusion products: (1) an RTK fused to a small fragment of .beta.-galactosidase and (2) a phosphotyrosine binding peptide fused to the large fragment of .beta.-galactosidase, where the 2 fragments weakly complex to form an active enzyme, and optionally a construct for a cytosolic RTK phosphorylating kinase, when the RTK does not autophosphoryate. To detect phosphorylation a .beta.- galactosidase substrate is added to the cells, whereby product formation indicates the occurrence of phosphorylation.