Modified human tumor necrosis factor receptor-1 polypeptide or fragment thereof, and method for preparing same

摘要

Provided is a modified human tumor necrosis factor receptor-1 polypeptide or a fragment thereof that binds to a tumor necrosis factor in vivo or ex vivo. The modified human tumor necrosis factor receptor-1 polypeptide or fragment exhibits improved ability to bind tumor necrosis factor and resistance to proteases.

主权项

1. A modified tumor necrosis factor receptor-1 polypeptide, comprising an amino acid sequence selected from the group consisting of:
an amino acid sequence comprising modifications of 5 amino acid residues consisting of 4 amino acid residues at positions 92, 95, 97 and 98, and 1 amino acid residue at one selected from among positions 68, 161, 200 and 207 in an amino acid sequence of a wild-type tumor necrosis factor receptor-1 polypeptide represented by SEQ ID NO: 1; an amino acid sequence comprising modifications of 5 amino acid residues consisting of 4 amino acid residues at positions 92, 95, 97 and 98, and 1 amino acid residue at one selected from among positions 68, 161, 200 and 207 in an amino acid sequence (TNFRI171) consisting of amino acids 41-211 of an amino acid sequence of a wild-type tumor necrosis factor receptor-1 polypeptide represented by SEQ ID NO: 1; an amino acid sequence comprising modifications of 5 amino acid residues consisting of 4 amino acid residues at positions 92, 95, 97 and 98, and 1 amino acid residue at one selected from among positions 68 and 161 in an amino acid sequence (TNFRI126) consisting of amino acids 41-166 of an amino acid sequence of a wild-type tumor necrosis factor receptor-1 polypeptide represented by SEQ ID NO: 1; and an amino acid sequence comprising modifications of 5 amino acid residues at positions 68, 92, 95, 97 and 98 in an amino acid sequence (TNFRI105) consisting of amino acids 41-145 of an amino acid sequence of a wild-type tumor necrosis factor receptor-1 polypeptide represented by SEQ ID NO: 1.