PRIMERS, SNP MARKERS AND METHOD FOR GENOTYPING MYCOBACTERIUM TUBERCULOSIS

摘要

The present application provides a primer set for genotyping M. tuberculosis selected from the group consisting of primer sets 1-25 (SEQ ID Nos. 1-50), and also provides an extension primer for genotyping M. tuberculosis selected from one of the group consisting of SEQ ID Nos. 51-75. The present application provides a combination of single-nucleotide polymorphism markers of M. tuberculosis. Further, the present application provides a method and a kit for genotyping M. tuberculosis.

主权项

1. A primer set for genotyping Mycobacterium tuberculosis selected from one of the group consisting of:Primer set 1:(SEQ ID No. 1)ACGTTGGATGTTCTGGACGACCTGTCCTACand(SEQ ID No. 2)ACGTTGGATGAGCTGCGCCAAGGTTCGTG,Primer set 2:(SEQ ID No. 3)ACGTTGGATGTTGTAGCTGCCCAAATTGCCand(SEQ ID No. 4)ACGTTGGATGGGCTTCAATCTCGGCTTGG,Primer set 3:(SEQ ID No. 5)ACGTTGGATGTATTCAACACCGGCATCGGGand(SEQ ID No. 6)ACGTTGGATGTCGCCTGGTCGTGGAAGAAC,Primer set 4:(SEQ ID No. 7)ACGTTGGATGATCGGACAGCAGAAGGCACand(SEQ ID No. 8)ACGTTGGATGACTCCCGCGGAACGTGGTG,Primer set 5:(SEQ ID No. 9)ACGTTGGATGCAACACCGGCAACTTCAACand(SEQ ID No. 10)ACGTTGGATGAATTAGCGTCTCCTCCGTTG,Primer set 6:(SEQ ID No. 11)ACGTTGGATGTCGAACCCGCCGACAAATGand(SEQ ID No. 12)ACGTTGGATGTCGATTGGTCGCATGCACTG,Primer set 7:(SEQ ID No. 13)ACGTTGGATGAAACCTCGGCATAGGGATCG (SEQ ID No. 14)ACGTTGGATGTCGACAGGACTATTGGTAGC,and Primer set 8:(SEQ ID No. 15)ACGTTGGATGAAGACGACGGGCCGGATATGand(SEQ ID No. 16)ACGTTGGATGCGTCAAGAGCTTCCCAAATC,Primer set 9:(SEQ ID No. 17)ACGTTGGATGCATCCGGGAACACCGTAAACand(SEQ ID No. 18)ACGTTGGATGATCACCTTCTTATCGGGTGG,Primer set 10:(SEQ ID No. 19)ACGTTGGATGCCTGGATTTCAGATATTGCCand(SEQ ID No. 20)ACGTTGGATGTGGCCAGCCCTAGCAAGTC,Primer set 11:(SEQ ID No. 21)ACGTTGGATGAGAACAAACGCGGGATTCACand(SEQ ID No. 22)ACGTTGGATGTCTCCCGGAGATCACCATTC,Primer set 12:(SEQ ID No. 23)ACGTTGGATGGTTGTTTTTGGCCGGGCAGand(SEQ ID No. 24)ACGTTGGATGATCGAGCAGACTCAGCGCTT,Primer set 13:(SEQ ID No. 25)ACGTTGGATGTGCTACCGCCAATGTTCAACand(SEQ ID No. 26)ACGTTGGATGATGGCGTTGACATAACTCGG,Primer set 14:(SEQ ID No. 27)ACGTTGGATGATAGCAAGCACGATTGCGACand(SEQ ID No. 28)ACGTTGGATGACCCCCCGCTGAGGGCGTA,Primer set 15:(SEQ ID No. 29)ACGTTGGATGGATTCGATTGGGGAAACGGCand(SEQ ID No. 30)ACGTTGGATGTTCCACATTGGTGATCAGCG,Primer set 16:(SEQ ID No. 31)ACGTTGGATGCAAACGGCGTCACTTTGGTCand(SEQ ID No. 32)ACGTTGGATGTGAAATGTGGGCCCAAGACG,Primer set 17:(SEQ ID No. 33)ACGTTGGATGCGATTTCGATCGGGATGTTGand(SEQ ID No. 34)ACGTTGGATGCAATCACGATCCCCTCAATC,Primer set 18:(SEQ ID No. 35)ACGTTGGATGAGGCAAAGGAAAATCGACCGand(SEQ ID No. 36)ACGTTGGATGTTGACAAACTGAAACACCGC,Primer set 19:(SEQ ID No. 37)ACGTTGGATGACAACCGGCCGCAGCGTTTand(SEQ ID No. 38)ACGTTGGATGAAGAACACCGAAAGTGGCTG,Primer set 20:(SEQ ID No. 39)ACGTTGGATGTGCATTGGCCACTAAAGCTCand(SEQ ID No. 40)ACGTTGGATGTCGATGACTATCTGCGGATG,Primer set 21:(SEQ ID No. 41)ACGTTGGATGACCCATTTGCCGAACGTGTCand(SEQ ID No. 42)ACGTTGGATGTGCTTGGCGACTTTGTGCAG,Primer set 22:(SEQ ID No. 43)ACGTTGGATGAGCGTGAAGAAGACGACGAand(SEQ ID No. 44)ACGTTGGATGGTCTGTTGTCATTACGGGAG,Primer set 23:(SEQ ID No. 45)ACGTTGGATGACATCAGGTGATGGTCATGCand(SEQ ID No. 46)ACGTTGGATGCGAAGGGAACAATGGATGTG,Primer set 24:(SEQ ID No. 47)ACGTTGGATGTATGCCAACCGATTTGCCTGand(SEQ ID No. 48)ACGTTGGATGACATATTGTCCACCGCGTAG,andPrimer set 25:(SEQ ID No. 49)ACGTTGGATGTCTTGGCAGCGGCATGGACand(SEQ ID No. 50)ACGTTGGATGCCGAATTTCCAGTCTCACAG.