发明名称 |
PROCESS FOR PREPARING HUMAN RELAXIN-2 |
摘要 |
A process for preparing human relaxin-2 having the following amino acid sequence: A chain: (SEQ ID NO: 1) pGlu-Leu-Tyr-Ser-Ala-Leu-Ala-Asn-Lys-Cys-Cys-His- Val-Gly-Cys-Thr-Lys-Arg-Ser-Leu-Ala-Arg-Phe-Cys B chain: (SEQ ID NO: 2) Asp-Ser-Trp-Met-Glu-Glu-Val-Ile-Lys-Leu-Cys-Gly- Arg-Glu-Leu-Val-Arg-Ala-Gln-Ile-Ala-Ile-Cys-Gly- Met-Ser-Thr-Trp-Ser; comprising the following steps: providing the amino acids necessary for the synthesis of the A and B chains with usual protective groups, wherein the cysteines are employed as trityl-protected amino acids (L-Cys(Trt)-OH); effecting a chromatographic purification of the individual chains A and B after the solid state synthesis; followed by the simultaneous folding and combination of the individual chains A and B in ammonium hydrogencarbonate buffer at pH 7.9 to 8.4; and subsequent purification of the relaxin-2 formed. |
申请公布号 |
EP2739647(A1) |
申请公布日期 |
2014.06.11 |
申请号 |
EP20120741355 |
申请日期 |
2012.08.03 |
申请人 |
PHARIS BIOTEC GMBH |
发明人 |
FORSSMANN, WOLF-GEORG;DSCHIETZIG, THOMAS;STÄNDKER, LUDGER;ZGRAJA, ANDREAS;HIRSCH, JOCHEN |
分类号 |
C07K14/64 |
主分类号 |
C07K14/64 |
代理机构 |
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