Method for the cytological analysis of cervical cells

摘要

The invention provides for a diagnostic test to monitor cancer-specific genetic abnormalities to diagnose cervical cell disorders and predict which patients might progress to cancer. Genetic abnormalities are detected by identification in chromosomal copy number of chromosome 3 and chromosome 5 using FISH analysis of probes targeted to 3q and/or 5p.

主权项

1. A method for detecting chromosomal abnormalities in a plurality of cells in a cervical sample, said method comprising:
a) hybridizing a first nucleic acid sequence to a target nucleic acid sequence on chromosome 3q; b) hybridizing a second nucleic acid sequence to a target nucleic acid sequence on a control chromosome; c) detecting a hybridization signal of the first and the second nucleic acid sequence, wherein the hybridization signal of said first and the second nucleic acid sequences is indicative of chromosomal copy number for chromosome 3q and the control chromosome; d) scoring the chromosomal copy number for chromosome 3q; and e) reporting whether the sample contains chromosomal abnormalities, wherein a lack of chromosomal abnormalities in the sample exists when a gain of 3q copy number is in less than 0.9% of the cells.