Delivery system for cell-based therapies

摘要

A warm perfusion system that has the ability to support a tissue or organ at a near normal metabolic rate provides the mechanism not only for restored oxidative metabolism of the organ but for the delivery of cells, cell-based therapeutics, and growth and differentiation factors to a damaged tissue or organ. Subsequent to delivery of therapeutic cells, such as stem cells or progenitor cells, to the damaged organ, the cells can be prompted to grow, multiply and differentiate, thereby restoring damaged tissue.

主权项

1. A method for the delivery of cell-based therapeutic agent to an ischemically damaged target tissue or organ, the method comprising:
(a) isolating the ischemically damaged target tissue or organ from the circulatory system of a body; (b) flushing the ischemically damaged tissue or organ with a non-blood buffered physiological solution to remove blood and blood products; (c) perfusing the tissue or organ in a recirculating warm perfusion solution capable of maintaining the tissue or organ at a near-normal metabolic rate; (d) introducing cells and/or a cell-based therapeutic agent into the perfusion solution; (e) perfusing the tissue or organ for a time sufficient for cells to become engrafted in the tissue or organ.