发明名称 Process for the fermentative preparation of L-ornithine
摘要 A process for the fermentative preparation of L-ornithine using microorganisms characterized by an increased export of the amino acid.
申请公布号 US8741608(B2) 申请公布日期 2014.06.03
申请号 US201113074458 申请日期 2011.03.29
申请人 Evonik Degussa GmbH 发明人 Claes Wilfried;Gerstmeir Robert
分类号 C12P13/10 主分类号 C12P13/10
代理机构 代理人
主权项 1. A process for preparing L-ornithine, comprising: a) cultivating in a medium an L-ornithine excreting bacterium selected from the group consisting of Corynebacterium, Bacillus, Streptomyces, Arthrobacter and the Enterobacteriaceae which overexpresses a polynucleotide coding for a polypeptide which has the activity of an L-ornithine exporter and whose amino acid sequence is identical to the amino acid sequence of SEQ ID No. 2 or comprises SEQ ID NO: 2 with a maximum of 25 deletions, insertions, substitutions or N-terminal and/or C-terminal additions of amino acids overall thus producing a fermentation broth, and b) recovering L-ornithine from the fermentation broth, wherein plasmid pEC7lysE, deposited as DSM23239, is excluded for overexpression.
地址 Essen DE