Métodos para obtener plantas resistentes a sequía

摘要

The present invention provides a nontoxic method for the selection of transformed cells from a cell population consisting of transformed and untransformed cells. The method comprises the following steps: a) introducing into a cell at least one nucleotide sequence of interest and at least one selection nucleotide sequence to obtain genetically transformed cells, where the selection nucleotide sequence comprises a sequence promoting the inhibition of endogenous trehalase enzyme; b) placing the population of transformed and untransformed cells in a culture medium containing an osmoregulating substance such as PEG 8000; c) selecting the transformed cells from the population based on the ability of transformed cells to survive and grow in the presence of the osmoregulating substance conferring the plants derived from these genetic transformation events, for example, drought and/or cold tolerance capacity.