| 摘要 |
The present invention relates to an aptamer-based isothermal nucleic acid amplification (APINA) which specifically combines with target materials and to a method for detecting ultra-high sensitive biomolecules using the same and, more specifically, to an aptamer-based isothermal nucleic acid amplification which specifically combines with physiological active substances and to a method for detecting DNA, protein, or physiological active substances with ultra-high sensitivity using the same. According to the present invention, the APINA solves problems which requires temperature changes of conventional nucleic acid amplificaltion method (PCR) which is the biggest obstacle for performing current nucleic acid test into field diagnosis techniques in order to have competitiveness in in-vitro diagnosis so that enormous creation of economic and industrial values can be expected, and is useful for detecting target materials (DNA, protein, and physiological active substances) with high sensitivity by applying the developed APINA to immune tests. |
