HIGH-AFFINITY TCR PROTEIN AND METHOD

摘要

PROBLEM TO BE SOLVED: To provide a T cell receptor (TCR) having higher affinity for a ligand than wild type TCR.SOLUTION: These high-affinity TCRs are obtained by steps of: mutagenizing a T cell receptor protein coding sequence to generate a variegated population of mutants of the T cell receptor protein coding sequence; transforming the T cell receptor mutant coding sequence into yeast cells; inducing expression of the T cell receptor mutant coding sequence on a surface of the yeast cells; and selecting the cells expressing T cell receptor mutants having higher affinity for a peptide/MHC ligand than wild type T cell receptor protein. The high-affinity TCR can be used in place of an antibody or single chain antibody.