METHOD FOR CLONING PLANT DISEASE RESISTANCE GENES IN HIGH-THROUGHPUT MANNER

摘要

Disclosed is a method for cloning plant disease resistance genes in a high-throughput manner. In the method, candidate disease resistance genes are obtained by means of bioinformatics; the candidate disease resistance genes are cloned in a high-throughput manner by using a high resistance variety or a relative species as a resistant gene source, and are transferred into a infected variety; and disease resistance capabilities of the genes are evaluated by using a method of pathogen infection, so as to ultimately find a broad-spectrum plant disease high resistance genes having importance production value. Further disclosed is a rice blast resistance gene obtained by using the method.