Measurement of 25-hydroxyvitamin D3 and C3-epi-25-hydroxyvitamin D3

摘要

The invention describes a method of quantification of an analyte selected from the group consisting of 25-hydroxyvitamin D3 and C3-epi-25-hydroxyvitamin D3 in a specimen containing the analyte; comprising the steps of subjecting the specimen to UPLC reverse-phase separation; and; detecting the protonated precursor pseudo-molecular ion of the analyte using a mass spectrometry technique to determine the amount of the analyte.