| 摘要 |
<p>When repeating a MS/MS analysis of the same test sample for a number of times, in which a precursor ion with a known m/z (m/z=M) is set, the MS/MS analysis is executed under dissociation conditions such that CID is unlikely to occur in some of the analytical runs (S3 to S9). When a MS/MS spectrum is created by integrating the spectrum data obtained in such a manner (S10), the precursor ion with a known m/z=M is always observed. Then, a peak corresponding to the ion in the MS/MS spectrum is detected, the mass deviation between the m/z measured value and the m/z theoretical value (M) is found (S11), and a spectrum is created for which other peak mass displacements are calibrated on the basis of the mass deviation (S12). Thus, mass calibration can be substantially performed with the internal standard method even for MS/MS spectra, and mass accuracy can be improved over conventional methods.</p> |
