摘要 |
[Abstract] [Problem] To provide a cell culture substrate, and a cell culturing method using the substrate and a method for inducing differentiation of pluripotent stem cells using 5 the substrate, which allow culturing of pluripotent stem cells and allow differentiation of pluripotent stem cells into a specified cell species, particularly neural and neural progenitor cells, at a high purity. [Means for Solution] A cell culture substrate, characterized in that, onto the surface, one or more selected from the group consisting of N-cadherin, a fusion protein 10 comprising an entire or partial region of N-cadherin, and a fusion protein comprising an entire or partial region of a protein homologous to N-cadherin are immobilized or coated. Fig. 1 ............. X.. ....... tEAK ~ ~ ~ %o~............. N <* ...... M ff.>y W N...... ......1 lzsdmm ...... ..... I N~~~~~~~~~2~~.. ......t:~ te {JO 14 44 e: 44 .. ........O F .. .. .............. . . .. .. . ...... ...... ............ ......................................sN2 .. ... .. -t 1 ......... .... .... .... ... .... .. : I , , " tr - : |