发明名称 |
CRYSTAL STRUCTURES OF HETERODIMERIC FC DOMAINS |
摘要 |
<p>Disclosed are the atomic coordinates of compositions comprising Fc heterodimer proteins in crystalline form derived from high resolution x-ray diffraction. Further disclosed are systems and methods for using all or a portion of these atomic coordinates to identify and design improved Fc heterodimer proteins. Further disclosed are compositions comprising a mixture of (i) a solubilized Fc heterodimer protein and (ii) a mother liquor solution. The mother liquor solution comprises between 2% and 10% (v/v) ethylene glycol, between 10% and 25% (w/v) polyethylene glycol having an average molecular weight of between 2000 Daltons and 10000 Daltons, and between 0.05 M and 0.40 M ammonium iodide. Further disclosed are systems and methods of identifying a mutation which promotes heterodimeric Fc chain pair formation in which structure based modeling is performed to identify a candidate mutation to an Fc chain using all or a portion of the disclosed three-dimensional atomic coordinates.</p> |
申请公布号 |
CA2889951(A1) |
申请公布日期 |
2014.05.08 |
申请号 |
CA20132889951 |
申请日期 |
2013.10.31 |
申请人 |
ZYMEWORKS INC. |
发明人 |
SPRETER VON KREUDENSTEIN, THOMAS;DIXIT, SURJIT BHIMARAO;LARIO, PAULA IRENE;ESCOBAR-CABRERA, ERIC;BOULANGER, MARTIN J.;SUITS, MICHAEL D.L. |
分类号 |
C07K16/46;C07K16/00;G01N33/68;G06F19/16 |
主分类号 |
C07K16/46 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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