| 摘要 |
Protein bound and ionic portions of an element contained in a sample are separated by depositing the sample on a support, drying the sample and coagulating the protein of the sample, subjecting the sample to electrophoresis to remove the ionic portion of the element, exposing the remainder of the sample to an oxidizing atmosphere in a vessel that also contains a liquid, heating the remainder of the sample for destruction of the protein so that the previously protein-bound element is released and absorbing said element in said liquid and then analysing the resulting liquid to determine the amount of protein-bound element. In each of the above steps the sample is treated on the support. Protein-bound iodine in blood serum, or phosphorus, iodine or sulphur in other samples can be analysed by this method. According to a preferred embodiment a plurality of supports in the form of closely wound platinum coils are mounted in a head which may be tilted to immerse the coils in a body of deionized water for the electrophoresis step. A plurality of electrical contacts is associated with each support whereby the coil may be heated to dry the sample, subject it to electrophoresis and finally decompose it. To carry out a determination, deionized water is placed in a container and electrical energy is applied individually by means of a selector switch to the coils to clean them; a suitable amount of sample is then applied to each coil. Relatively low electric power is applied simultaneously to the coils to dry the samples, then the assembly is tilted downwards so that the coils extend into the deionized water and a direct current is applied between the coils and container to remove the non-protein-bound element electrophoretically; as the head is thereafter tilted upwards low power electrical energy is applied simultaneously to the various coils to dry the material remaining on them. Flasks containing oxygen and partially filled with water are placed over each of the coils which are then energized to burn the remaining sample; the combustion products are absorbed in the water and the amount of previously protein-bound element determined colorimetrically. |
