FLAVIN-BOUND GLUCOSE DEHYROGENASE, PRODUCTION METHOD FOR FLAVIN-BOUND GLUCOSE DEHYROGENASE, AND YEAST TRANSFORMANT USED IN SAME

摘要

The present invention provides heat-resistant flavin-bound glucose dehydrogenases having high substrate specificity for D-glucose, an efficient production method of the same, and a transformant used for the same. A flavin-bound glucose dehydrogenase gene encoding a flavin-bound glucose dehydrogenase derived from Mucor is introduced into yeast, Zygosaccharomyces, to obtain a transformant. Subsequently, the yeast transformant is cultured to obtain a flavin-bound glucose dehydrogenase from the culture. A heat-resistant flavin-bound glucose dehydrogenase that is less susceptible to the effects of dissolved oxygen and allows accurate measurement of glucose even in the presence of sugar compounds other than glucose in a sample can be efficiently produced.