| 摘要 |
Method for enzymatically synthesising a peptide, comprising enzymatically preparing an ester or a thioester from: (i) an N-terminal protected amino acid, an N-terminal protected amino acid C-terminal ester, an N-terminal protected peptide, or an N-terminal protected peptide C-terminal ester; and (ii) an alcohol represented by the formula HO-CX-Z respectively a thiol represented by the formula HS-CX-Z, each X independently representing a halogen atom or a hydrogen atom; and Z being selected from the group of sp-hybridised carbons comprising at least two substituents comprising a heteroatom directly attached to the sp-hybridised carbon and sp-hybridised carbons comprising one or two substituents comprising a heteroatom directly attached to the sp-hybridised carbon, the preparation of the ester or thioester being carried out in a reaction medium comprising 2 wt.% water or less based on the weight of liquids in the reaction medium; and enzymatically coupling the prepared ester or thioester with an, optionally C-terminal protected, amino acid or with an, optionally C-terminal protected, peptide, thereby synthesising the peptide in a reaction medium comprising 2 wt.% water or less based on the total weight of the reaction medium. |
