PROCESSOS E KITS PARA SINTETIZACAO E AMPLIFICAÇÃO DO ACIDO NUCLEICO BEM COMO, PROCESSOS PARA DETECTAR UMA SEQUENCIA DE NUCLEOTIDEO ALVO E DE UMA MUTACAO DA DITA SEQUENCIA

摘要

An oligonucleotide having a novel structure and a method for synthesizing a nucleic acid by using the same as a primer. In the 5'-side of the primer, this oligonucleotide is provided with a base sequence which is substantially identical with the region to be synthesized by using this primer as a starting point of the synthesis. Thus, a nucleic acid can be synthesized on the basis of an isothermal reaction with the use of a simple reagent constitution. Also, provision is made of a method for synthesizing a nucleic acid having a high specificity based on the above method for synthesizing a nucleic acid.