| 摘要 |
PROBLEM TO BE SOLVED: To provide a method of preparing a tissue implant for use in augmenting, repairing or regenerating tissue.SOLUTION: A method of preparing a tissue implant in vitro, said method comprising: (a) providing cells isolated from a suitable tissue source; (b) seeding at least two primary scaffolds formed of polymeric fibers with the cells and culturing these scaffolds for a period of time sufficient for the cells to secrete an extracellular matrix and form a cellular aggregate and; (c) loading the at least two scaffolds obtained in (b) into a secondary scaffold formed of polymeric fibers. |
