CDKACT BIOSENSORS-FLUORESCENT POLYPEPTIDE TO PROBE THE ACTIVITY OF CDK/CYCLIN KINASES IN VITRO, IN CELLULO AND IN VIVO

摘要

This invention relates to compounds comprising a polypeptide and a fluorophore, wherein the polypeptide is capable of being phosphorylated by a Cyclin-Dependent Kinase (CDK) and/or a Cyclin-CDK complex on a specific site and wherein the fluorescence of said fluorophore changes upon phosphorylation of said specific site of said polypeptide. The invention also relates to the use of such compounds or of compositions comprising such compounds for medical imaging. The invention furthermore relates to methods for determining if at least one CDK and /or Cyclin-CDK complex is active, for determining changes in at least one CDK and /or Cyclin-CDK complex activity, for the in vitro diagnosis of Cyclin-dependent kinase hyperactivation in a subject.