| 摘要 |
PROBLEM TO BE SOLVED: To manufacture a pigment-containing artificial skin capable of retaining, over an extended period, melanocytes effective for explorations of treatments of pigment freckle disorder.SOLUTION: The provided method for manufacturing a pigment-containing artificial skin comprises the following steps 1 through 3: (1) a step 1 of preparing a dermic layer by mixing a carrier and dermal fibroblasts and then incubating the resulting mixture; (2) a step 2 of disseminating and incubating melanocytes within the upper portion of the dermic layer prepared at the step 1; and (3): a step 3 of preparing an epidermal layer by disseminating and incubating epidermal keratinocytes within the upper portion of the dermic layer prevailing after the step 2. |
