SET OF SYNTHETIC OLIGONUCLEOTIDES FOR IDENTIFICATION OF DNA OF PARODONTOPATHOGENIC MICROORGANISM Candida albicans BY METHOD OF POLYMERASE CHAIN REACTION

摘要

FIELD: chemistry.SUBSTANCE: invention relates to field of biochemistry, in particular to set of synthetic oligonucleotides for identification of DNA of parodontopathogenic microorganism Candida albicans by method of polymerase chain reaction. Claimed set includes specific to fragment of gene gr1 of microorganism Candida albicans primers 5?-TTGCCATTCTTGGACGAAGG-3? and 5?-CAACAATGGCAACTTTTTTAGG-3?, as well as probe (BHQ1)-5?-TCCTCCTTCAG(FdT)CCCTGGTGCTGA-3?-P, where BHQ1 stands for attached to 5'-tail nucleotide blank fluorescence damper, FdT - fluorescent dye FAM, attached to T nucleotide.EFFECT: claimed invention represents an effective marker for identification of microorganism Candida albicans presence in biological material.