| 摘要 |
<p>The present invention provides a method for discriminating single nucleotide polymorphisms (SNPs) which offers improved sensitivity and specificity. The invention relates also to a test kit. Thus, the present invention is a method for genotyping in typing assays applied to a biological sample, the sample containing one nucleic acid target of interest, the target nucleic acid being susceptible to contain a single nucleotide polymorphism (SNP), comprising the steps of:
- performing a real-time amplification of the target, generating multiple copies of amplicons, in presence of at least two different labeled probes, each probe allowing real-time detection at the SNP position of both the wildtype and at least one possible mutation,
- assessing the discriminatory variable value(s) based on the signals of each combination of two detection probes, and
- discriminating between the genotypes. The present invention is especially useful in methods for diagnostic, preventive and therapeutic applications.</p> |
