METHOD OF PRODUCING CONTINUOUS CELL LINES AND USE THEREOF

摘要

FIELD: chemistry.SUBSTANCE: invention relates to biotechnology and a method of producing continuous lines of living cells and use thereof. The disclosed method involves irradiating said living cells with a UV light dose of about 20 mJ/cmto about 300 mJ/cm, at a wavelength between about 100 nm and about 400 nm, for about 30 s to 5 min and collecting cells that are capable of proliferation after at least 20 passages. The obtained cell lines are deposited in ECACC under numbers 08020602, 08020603 and 08020604 and can be used to produce viruses or gene products.EFFECT: invention facilitates recombinant expression of biomolecules.23 cl, 4 dwg, 1 tbl, 6 ex