发明名称 |
USE OF ZSCAN4 AND ZSCAN4-DEPENDENT GENES FOR DIRECT REPROGRAMMING OF SOMATIC CELLS |
摘要 |
<p>Disclosed herein is the finding that Zscan4 is an early embryonic factor that facilitates cellular reprogramming. In particular, Zscan4 can replace the oncogenic reprogramming factor c-Myc to produce induced pluripotent stem cells when co-expressed with Klf4, Oct4 and Sox2. In addition, several Zscan4-dependent genes were identified that promote iPSC formation when co-expressed with known reprogramming factors. Thus, the present disclosure provides an ex vivo method of producing an iPS cell by reprogramming of a somatic cell. The method includes contacting the somatic cell with a Zscan4, or a Zscan4-dependent gene, and at least one reprogramming factor. Also provided are iPS cells produced by the disclosed method and non-human animals generated from such iPS cells.</p> |
申请公布号 |
EP2707479(A1) |
申请公布日期 |
2014.03.19 |
申请号 |
EP20120785169 |
申请日期 |
2012.05.11 |
申请人 |
THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES |
发明人 |
KO, MINORU, S.H.;HIRATA, TETSUYA |
分类号 |
C12N5/00;C12N5/074;C12N5/10;C12N15/00;C12N15/63;C12N15/85 |
主分类号 |
C12N5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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