发明名称 |
Method of Genome Surgery with Paired, Permeant Endonuclease Excision |
摘要 |
The use of P2E2 constructs in genome surgery includes a cell penetration component, a DNA binding component and a restriction endonuclease. The method for performing genome surgery includes: a) providing one or more recombinant of the P2E2 constructs; b) penetrating a cell with the recombinant P2E2 protein construct; c) forming a protein product in the cell by the processes of transcription and translation or by direct introduction of the P2E2 protein construct to the cell; d) attaching the protein product of the P2E2 construct to one or more targeted genomic sequences within the cell; and e) the endonuclease of the P2E2 construct cutting both strands of the genome at target locations. |
申请公布号 |
US2014072961(A1) |
申请公布日期 |
2014.03.13 |
申请号 |
US201313937860 |
申请日期 |
2013.07.09 |
申请人 |
UNIVERSITY OF NEVADA, LAS VEGAS |
发明人 |
SCHILLER MARTIN;STRONG CHRISTY |
分类号 |
C12N15/52 |
主分类号 |
C12N15/52 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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