摘要 |
A method for counting microorganisms present in a biological sample in contact with a culture medium adapted to the growth of said microorganisms, Number NSA of microorganisms present in the sample is determined at a prior stage, Number NSU of microorganisms present at a subsequent stage is calculated according to number NSA. The calculation is based on a model of microorganism growth in the culture medium according to: log(NSU)=alpha�log(NSA)-beta�log(CSA)+gamma, where log is the decimal logarithm, NSU is the calculated number of microorganisms, NSA is the number of microorganisms at the prior stage, CSA is the number of microorganisms at the prior stage divided by the volume of the sample, and alpha, beta, and gamma are determined parameters depending on the microorganisms, the culture medium, and the time period separating the subsequent stage from the prior stage, alpha and beta being positive. |