| 摘要 |
FIELD: biotechnologies.SUBSTANCE: as per the first version, a method is implemented by recording of cyclic voltamperograms of a working electrode modified with carbon nanotubes with a oligonucleotide probe noncovalently immobilised on their surface, before and after the nucleic acid specimen is added to the investigated solution, and as per the change of capacitive characteristic, it is evaluated whether a section complementary to the oligonucleotide probe is available in the specimen or not. The second version of the method differs by the fact that non-covalent immobilisation of the oligonucleotide probe onto the surface of nanotubes is performed by means of an anchor group pre-introduced to the probe. This version includes recording not only of the change of surface area of voltamperograms from cycle to cycle, but also occurrence of a specific peak on a cyclic voltamperogram, which is related to fixation of detected nucleic acid complete with the modified probe. Intensity of the peak on the cyclic voltamperogram is pro rata to concentration of the determined nucleic acid, which allows performing quantitative evaluation. A device for implementation of the detection method of specific sequences of nucleic acids represents an electrochemical analyser that consists of a three-electrode electrochemical cell, the electrodes of which are connected to a recording device, and the working electrode is made from a silicone substrate modified with vertically oriented carbon nanotubes with an immobilised oligonucleotide probe complementary to the nucleic acid to be determined.EFFECT: improving evaluation accuracy.8 cl, 5 dwg, 4 ex |
