IMMUNOLOGICAL DETECTION METHODS AND COMPOSITIONS

摘要

A flexible method for using bioluminescence to enhance the detection and sensitivity of immunoassays in the detection of any analyte. The method of the present invention comprises the use of antibodies attached to luciferase to enhance detection and sensitivity of immunoassays. Detection can be enhanced by use of methods that comprise incubation of a substrate or a set of beads coated with a dockerin or a cohesin protein with a cohesin or dockerin-antigen fusion proteins to attach the fusion protein essentially irreversibly by non- covalent interaction in addition to antibodies attached to luciferase.