A method and kit is provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minize interference by at least about 10% relative to an assay not having tolerance enhancement agent.
申请公布号
EP2325328(B1)
申请公布日期
2014.02.12
申请号
EP20100075525
申请日期
2003.12.23
申请人
PROMEGA CORPORATION
发明人
HAWKINS, ERIKA;CALI, JAMES J.;HO, SAMUEL KIN SANG;O'BRIEN, MARTHA;SOMBERG, RICHARD;BULLEIT, ROBERT F.;WOOD, KEITH V.