MUTANT MICROORGANISM HAVING IMPROVED PRODUCTION ABILITY OF 1,4-BDO AND METHOD FOR PREPARING 1,4-BDO USING THE SAME

摘要

One embodiment of the present invention relates to a metabolism network model of Corynebacterium glutamicum for producing 1,4-BDO by Corynebacterium glutamicum, which is a strain that does not produce 1,4-BDO in a natural state. On the basis of the network model, metabolic properties of Corynebacterium glutamicum are analyzed. By predicting effects of a deletion target enzyme (ldhA, mqo or mdh) in a state in which Cat1, sucD, 4hbD, cat2 and adhE are included, and removing them, production efficiency of 1,4-BDO and increase of 1,4-BDO under the same fermentation conditions are confirmed. In addition, transformation microorganism produced by the method can produce 1,4-BDO in high efficiency, and therefore, can be effectively used in the industries.