| 摘要 |
There is described a mutant, non-naturally occurring or transgenic plant or plant cell comprising (a) a polynucleotide selected from the group consisting of: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence having at least 71 % sequence identity to SEQ ID NOs: 1, 2, 27, 28 or 29 or 51; or (ii)a polynucleotide comprising, consisting or consisting essentially of a sequence having at least 65 % sequence identity to any of SEQ ID NOs: 3 to 23 or 30 to 50; or (iii) a polynucleotide encoding a NtMRP polypeptide comprising, consisting or consisting essentially of a sequence having at least 65% sequence identity to any of SEQ ID NOs. 24 to 26 or 52, and wherein the polypeptide has heavy metal transporter activity; or (b) a polynucleotide construct of at least 15 contiguous nucleotides in length that is at least 65 % identical to a region of any of SEQ ID NOs: 1 to 23 or 27 to 51; or (c) a double-stranded RNA comprising at least two sequences that are at least partially complementary to each other and wherein a sense strand comprises a first sequence and an antisense strand comprises a second sequence and wherein at least one of the sequences comprises at least 10 contiguous nucleotides of NtMRP RNA; or (d) an expression vector comprising the polynucleotide as set forth in (i), (ii) or (iii) or the polynucleotide construct as set forth in (b). |
