| 摘要 |
This invention relates to a thermophilic polymerase wherein the polymerase has an in vitro primer extension rate that is > 35 bases/second and faster relative to the primer extension rate of a DNA polymerase comprising amino acid sequences SEQ ID NO: 2 or 4, when measured under identical conditions in a DNA replication assay using primed single strand M13mp18 DNA and an incubation temperature of 60°C. The invention further relates to chimeric polymerases, wherein the chimeric polymerase may comprise the complete or partial amino acid sequence of SEQ ID NO: 6. The invention also relates to nucleic acid sequences encoding the polymerase according to the invention as well as vectors comprising a nucleic acid encoding the polymerase according to the invention. |
