发明名称 |
FRAGMENT COMPLEMENTATION ASSAYS FOR G-PROTEIN-COUPLED RECEPTORS AND THEIR SIGNALING PATHWAYS |
摘要 |
<p>The invention provides a method of screening a candidate drug, a compound library or a biological extract to identify activators or inhibitors of G-protein-coupled receptors or G-protein-coupled pathways, comprising: (A) using a fluorescent protein fragment complementation assay to construct an assay for one or more steps in a G-protein-coupled pathway; (B) testing the effects of the candidate drugs, compound library, or biological extract on the receptor or pathway of interest; and (C) using the results of the screening to identify specific agents that activate or inhibit the receptor or pathway of interest. The invention also provides a method for identifying a drug lead that modulates the activity of a G-protein-coupled pathway using a fluorescent protein fragment complementation assay. The method of the invention is used to identify agonists, antagonists, activators or inhibitors of G-protein coupled receptors or G-protein-coupled pathways.</p> |
申请公布号 |
EP1668161(B1) |
申请公布日期 |
2014.01.22 |
申请号 |
EP20040785127 |
申请日期 |
2004.09.24 |
申请人 |
ODYSSEY THERA INC. |
发明人 |
WESTWICK, JOHN, K.;KEON, BRIGITTE;MACDONALD, MARNIE, L. |
分类号 |
C12Q1/68;C07K16/28;C12P21/02;C12Q1/00;G01N;G01N21/68;G01N33/50;G01N33/53;G01N33/543;G01N33/566;G01N33/58;G01N33/74 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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