PRODUCTION OF HIGHLY PURIFIED SODIUM HYALURONATE (HANA) WITH CONTROLLED MOLECULAR WEIGHT

摘要

Disclosed is a process for the production of sodium hyaluronate with a molecular weight of between 60 and 2400 kDa and low polydispersity (1.4Mw/Mn), which comprises: a) a step of fermentation of Streptococcus equi subsp. zooepidemicus CNCM I-4645 in a suitable culture medium; b) a step of ultrafiltration of the cell-free filtered solution, by concentrating and diafiltering the solution under differential pressure conditions (∆P) of 1.0-5.0 barg and transmembrane pressure (TMP) of 0.5-4 barg.