HEAVY METAL-CONTAMINATED SOIL WASHING METHOD USING SOIL WASHING SOLUTION INCLUDING SIDEROPHORE AND HEAVY METAL-CONTAMINATED SOIL WASHING DEVICE

摘要

The present invention relates to a heavy metal-contaminated soil washing method using soil washing solution including siderophore and a heavy metal-contaminated soil washing device which is based on the principle that siderophore solubilizes heavy metal by reaction with an insoluble compound including heavy metal or forms a soluble siderophore-heavy metal complex by the direct combination of siderophore and heavy metal. The heavy metal-contaminated soil washing method according to the present invention comprises: a first step for generating siderophore by culturing Pseudomonas aeruginosa in a nutrient solution; a second step for obtaining a first soil washing solution including siderophore by removing Pseudomonas aeruginosa from the nutrient solution; a third step for crushing heavy metal-contaminated soil; a fourth step for washing the soil by mixing the crushed soil with the first soil washing solution; and fifth step for separating, by centrifugation, the first soil washing solution from the soil mixed with the first soil washing solution, wherein in the fourth step, siderophore dissolves heavy metal in the first soil washing solution by reaction with the insoluble compound including heavy metal or dissolves heavy metal by forming the soluble siderophore-heavy metal complex via the direct combination with the heavy metal while the crushed soil is mixed with the first soil washing solution. [Reference numerals] (AA) Start; (BB) End; (S10) Generate siderophore by culturing Pseudomonas aeruginosa in a nutrient solution; (S20) Obtain a first soil washing solution by removing Pseudomonas aeruginosa from the nutrient solution; (S30) Crush heavy metal-contaminated soil; (S40) Wash the soil by mixing the crushed soil with the first soil washing solution; (S50) Separate the first soil washing solution from the soil by centrifugation