KIT FOR ISOTHERMAL DNA AMPLIFICATION STARTING FROM AN RNA TEMPLATE

摘要

A kit for amplifying deoxynucleic acid (DNA) from ribonucleic acid (RNA) template is provided. The kit for amplifying a RNA comprises at least one inosine-containing primer; and at least one enzyme comprising a reverse transcriptase activity, a strand displacement DNA polymerase activity, a nuclease acitivity for nicking DNA 3' to an inosine residue of the primer or combinations thereof. The kit further comprises one or more quantifying reagents to detect the presence of RNA in a sample or quantify the RNA present in a sample.