METHOD AND APPARATUS FOR PERFORMING QUANTITATIVE ANALYSIS OF NUCLEIC ACID USING REAL-TIME PCR

摘要

A method and apparatus for performing quantitative analysis of a nucleic acid by determining a curve-fitting area based on fluorescence intensity data obtained by performing PCR on a target nucleic acid; analyzing parameters for amplification efficiency and nucleic acid concentration by curve-fitting a result of performing PCR on a reference nucleic acid with a known initial nucleic acid concentration; and estimating the initial nucleic acid concentration of the target nucleic acid by performing curve-fitting on the determined curve-fitting area using the analyzed parameters.