发明名称 |
Use of glycosaminoglycans to reduce non-specific binding in immunoassays |
摘要 |
An immunoassay reagent is provided which comprises an analyte binding agent in a diluent, and a glycosaminoglycan in an amount sufficient to reduce non-specific binding in an assay of a sample for the analyte. Provided is such an immunoassay reagent in which the analyte is troponin I, the analyte binding agent is a biotinylated anti-troponin I antibody, and the glycosaminoglycan is chondroitin sulfate. A sample composition is also provided which comprises a sample to be assayed for the presence of an analyte, an analyte binding agent, and a glycosaminoglycan other than heparin. Further provided is a method of detecting an analyte in a sample, in which non-specific binding is reduced in the method using a glycosaminoglycan. |
申请公布号 |
US8617820(B2) |
申请公布日期 |
2013.12.31 |
申请号 |
US20070846390 |
申请日期 |
2007.08.28 |
申请人 |
SANKARAN BANUMATHI;SULLIVAN SHERYL S.;HAYNES DARRELL C.;HOSIMER PHILIP C.;YEARWOOD GRAHAM;ORTHO-CLINICAL DIAGNOSTICS, INC. |
发明人 |
SANKARAN BANUMATHI;SULLIVAN SHERYL S.;HAYNES DARRELL C.;HOSIMER PHILIP C.;YEARWOOD GRAHAM |
分类号 |
C12Q1/00;C12N5/07;G01N33/00;G01N33/53 |
主分类号 |
C12Q1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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