SYNTHETIC ANTIGEN HAVING ABILITY TO FIX AUTOANTIBODIES TO MUSCARINE M2-RECEPTOR

摘要

FIELD: biotechnologies.SUBSTANCE: invention describes creation of an artificial conformational antigen including peptide fragments consisting of two extracellular loops of muscarine M2-receptor, which are cross-linked with disulphide bond, and which is able to fix autoantibodies to this receptor with sensitivity exceeding the sensitivity for a linear prototype or mechanical mixture of data of linear peptide fragments. Synthetic antigen represents an individual chemical compound containing peptide corresponding to the sequence of the 1-st extracellular loop and peptide of the 2-nd extracellular loop, which are linked with disulphide connection between Cysand Cysresidues (structure of antigen is disclosed in the formula and description). As per the invention, there described are methods for obtaining and using an antigen for creation of a diagnostics set for reveal of antibodies to muscarine M2-receptor in blood of patients with idiopathic arrhythmia using an enzyme-linked immunosorbent assay (ELISA) method.EFFECT: improvement of the method.5 cl, 1 dwg, 3 tbl, 4 ex