发明名称 |
DETECTING MUTATIONS IN DNA |
摘要 |
Provided herein are methods for detecting mutations in nucleic acid, and compositions and kits for performing such methods. In particular, nucleic acid amplification and fluorescence detection methods are provided to detect mutations and assess the mutational load. The methods are based on a set of adjacently binding probes wherein one probe is labelled with a quencher and the other is a self-indicating probe labelled with fluorophore and quencher. The binding of the probes is analysed by melting curve analysis. |
申请公布号 |
US2013344484(A1) |
申请公布日期 |
2013.12.26 |
申请号 |
US201113991022 |
申请日期 |
2011.12.01 |
申请人 |
OSBORNE ADAM E.;WANGH LAWRENCE J.;RICE JOHN E.;BRANDEIS UNIVERSITY |
发明人 |
OSBORNE ADAM E.;WANGH LAWRENCE J.;RICE JOHN E. |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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