发明名称 |
Methods for sequencing a nucleic acid using large scale FET arrays, configured to measure a limited pH range |
摘要 |
A method for sequencing a nucleic acid may include (i) disposing a plurality of template nucleic acids into reaction chambers that are in contact with a chemical-sensitive field effect transistor (chemFET) array including a chemFET for each reaction chamber, (ii) synthesizing a new nucleic acid strand by incorporating one or more known nucleotide triphosphates sequentially at the 3' end of sequencing primers hybridized to the template nucleic acids, and (iii) detecting the incorporation of the one or more known nucleotide triphosphates by a change in current at the at least one chemFET within the array. The chemFET has a floating gate, is sensitive to ions, and is part of a pixel that is configured to measure a limited pH value. |
申请公布号 |
EP2677309(A1) |
申请公布日期 |
2013.12.25 |
申请号 |
EP20130177039 |
申请日期 |
2007.12.14 |
申请人 |
LIFE TECHNOLOGIES CORPORATION |
发明人 |
ROTHBERG, JONATHAN M.;HINZ, WOLFGANG;JOHNSON, KIM L.;BUSTILLO, JAMES |
分类号 |
G01N27/414;C12Q1/68;G01N33/543;H01L27/088;H01L29/78 |
主分类号 |
G01N27/414 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|