发明名称 |
OLIGONUCLEOTIDE-MEDIATED QUANTITATIVE MULTIPLEXED IMMUNOASSAYS |
摘要 |
Methods and compositions for quantitative immunoassays are provided, in which ligand-conjugated probes are used to label samples and ligand-surfaced microspheres are used as quantitative reference standards. Certain embodiments provide a method of quantitative flow cytometry where ligands are oligonucleotides, and a sample comprising one or more cells is contacted with a hybridized antibody: :fluorophore labeled targeting construct to label the cells, and the labeled cells are analyzed. In some embodiments, a population of quantitative labeled oligospheres labeled with the same fluorescent label as the cells is analyzed using the flow cytometer and used to create a quantitative standard curve of cytometer intensity versus molecules fluorescent label per oligosphere event. A standard curve trendline is established and used to determine the molecules of fluorescent label per cellular event for the antigen-positive cell populations. Based on molecules of fluorecent label per cell, the amount of Antibody Binding per Cell (ABC) is quantified. |
申请公布号 |
WO2013188756(A1) |
申请公布日期 |
2013.12.19 |
申请号 |
WO2013US45872 |
申请日期 |
2013.06.14 |
申请人 |
THE UNIVERSITY OF CHICAGO |
发明人 |
FLOR, AMY;DUGGAN, RYAN;KRON, STEVE |
分类号 |
G01N33/53 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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地址 |
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