发明名称 |
METHOD FOR PRODUCING VIRUS VECTOR FOR GENE TRANSFER |
摘要 |
The present invention discloses a cell system, as a host cell to be infected with an F gene-deficient virus, which can constitutively and stably express the F protein, and a method for producing an F gene-deficient virus by utilizing the cell. A non-proliferative human parainfluenza type 2 virus vector is produced by co-culturing an F gene-deficient human parainfluenza type 2 virus with a Vero cell having the F gene of human parainfluenza type 2 virus in such a manner that the F gene is non-inducibly expressed, and isolating viral particles from a culture supernatant. |
申请公布号 |
EP2674491(A1) |
申请公布日期 |
2013.12.18 |
申请号 |
EP20120744339 |
申请日期 |
2012.02.08 |
申请人 |
MIE UNIVERSITY;BIOCOMO INC. |
发明人 |
FUKUMURA, MASAYUKI;KAWANO, MITSUO;NOSAKA, TETSUYA;OHTSUKA, JUNPEI |
分类号 |
C12N15/09;A61K35/76;A61P37/04;C12N5/10 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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