发明名称 |
MODIFIED RNASE H AND DETECTION OF NUCLEIC ACID AMPLIFICATION |
摘要 |
A reversibly modified 'hot start' RNAse H enzyme composition is described for the improved CATACLEAVE(TM) probe detection of nucleic acid sequences in a test sample. A key feature of the enzyme composition is the ability to regulate the catalytic activity of the RNAse H during the course of a reverse transcription-PCR cycle. Thus, RNAse H activity can be initially suppressed to minimize degradation of RNA:DNA primer heteroduplexes prior to reverse transcription. After cDNA synthesis is complete, RNAse H activity is induced to promote the cleavage and fluorescent detection of CATACLEAVE(TM) probes that anneal to target DNA sequences within the reverse transcriptase-PCR products. The inducible RNAse H enzyme is amenable to high throughput applications requiring one step reverse transcriptase CATACLEAVE(TM) PCR in a single reaction mix. |
申请公布号 |
EP2576774(A4) |
申请公布日期 |
2013.12.11 |
申请号 |
EP20110786887 |
申请日期 |
2011.05.25 |
申请人 |
SAMSUNG TECHWIN CO., LTD |
发明人 |
CHEUNG, WIN DEN;OPDYKE, JASON |
分类号 |
C12N9/22;C12N15/10;C12Q1/34;C12Q1/68 |
主分类号 |
C12N9/22 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|