METHOD FOR AFFINE SEPARATION OF AUTOANTIBODIES OF IgG CLASS TO IMMUNOREGULATORY CYTOKINE TNF

摘要

FIELD: biotechnologies.SUBSTANCE: invention refers to a separation method of fractions of autoantibodies of IgG class to immunoregulatory cytokine TNF from human blood serum using a set of affine chromatography procedures and a magnetic separation procedure performed using magnetic particles covered with streptavidin, and polyclonal antibodies to TNF, which are marked with biotin.EFFECT: obtaining pure fractions of autoantibodies to TNF of IgG class.1 cl, 1 ex, 1 tbl