| 摘要 |
FIELD: medicine.SUBSTANCE: invention describes a method for alkaline phosphatase test in bone and cartilage tissues involving preparing the analysed tissue samples and conducting the histochemical examination of the material sections, wherein the bone and cartilage samples are kept in a buffered solution of 10% neutral formalin for 1 day at temperature +4°C, then processed in 0.1M solution of phosphate buffer first changed 4 times every 2 hours, and then changed 9 times every one hour with saccharose added in the gradually increasing concentration; thereafter the analysed material is immersed into Tissue-Tek O.C.T. Compound, frozen at -35°C; the sections are prepared and visually inspected for alkaline phosphatase.EFFECT: method is easy to implement; it is applicable for alkaline phosphatase test in the tissue samples conducted in every health care facility and research laboratory with using no special equipment.5 dwg, 1 ex |
