SEPARATION OF BINDING MOLECULES

摘要

A method of separating one or more binding molecules that are specifically bound to one or more binding targets from a mixture of bound and unbound molecules includes introducing the mixture into at least one inlet of a microfluidic device at a sample flow rate. The microfluidic device includes at least one curved channel, wherein each curved channel has a length, a radius, and a cross-section of a height and a width defining an aspect ratio adapted to isolate the one or more molecules bound to the binding target along portions of the cross-section of the channel based on size. Bound molecules flow along a radially innermost portion of the channel to a first outlet, and unbound molecules flow along one or more other portions of the channel to at least one other outlet, thereby separating the one or more binding molecules that are specifically bound to the one or more binding targets from the mixture of bound and unbound molecules.